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CSF collection

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CSF chemistry
CSF chemistry
Lumbar vertebrae
Lumbar vertebrae


CSF collection

Definition:

Cerebral spinal fluid (CSF) collection is a test to sample and measure pressure in the fluid that surrounds the brain and spinal cord. Cerebral spinal fluid acts like a cushion, protecting the brain and spine from injury. The fluid is normally clear.

See also CSF culture.

Text Continues Below



Alternative Names:
Spinal tap; Ventricular puncture; Lumbar puncture; Cisternal puncture; Cerebral spinal fluid culture

How the test is performed:

There are different ways to get a sample of spinal fluid. Lumbar puncture, commonly called a spinal tap, is the most common method. The test is usually performed in the following manner:

  • The patient must lay on his or her side, with the knees pulled up toward the chest, and the chin tucked downward. Sometimes the test is done with the person sitting up, but bent over. 
  • After the back is cleaned, the health care provider will inject  a local numbing medicine (anesthetic) into the lower spine.
  • A spinal needle is inserted, usually between the 3rd and 4th lumbar vertebrae.
  • Once the needle is properly positioned, spinal fluid pressure is measured, and fluid is collected.
  • The needle is removed, the area is cleaned, and a bandage is placed over the needle site. The patient will need to remain flat for 20 minutes to 1 hour after the test.

Lumbar puncture with fluid collection may also be part of other procedures, particularly a myelogram (x-ray or CT scan after dye has been inserted into the CSF).

Alternative methods of CSF collection are rarely used, but may be necessary in in the event of a back deformity or infection.

Cisternal puncture uses a needle placed below the occipital bone (back of the skull). It can be dangerous because it is so close to the brain stem.

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