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Blood collection:

Blood is drawn from a vein on the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and an elastic band is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the band to fill with blood.

A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

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For an infant or young child, the area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.

How to prepare for the test:

Fast for 8 to 12 hours before the test. Consult the health care provider about the need to withhold drugs that can affect test results (see "special considerations").

If the collection is being taken from an infant, a couple of extra collection bags may be necessary.

For infants and children:

The preparation you can provide for this test depends on your child's age and previous experience. For specific information regarding how you can prepare your child, see the following topics:

How the test will feel:

When the needle is inserted to collect the blood, a stinging sensation or prick will be felt. After the blood has been drawn, the puncture site may throb for a few minutes, and may feel bruised for a day or two.

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