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CSF culture

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Pneumococci organism
Pneumococci organism


CSF culture

Definition:

CSF culture is a test used to detect the presence of microorganisms (bacteria, viruses, and fungus) in the cerebrospinal fluid (CSF) causing infection. CSF is a clear fluid that circulates in the space surrounding the spinal cord.

The CSF protects the brain and spinal cord from injury and physical shocks, and carries products of neurosecretions (chemicals released by the neural tissue), chemicals in the cells, and chemical changes in the cells.

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Alternative Names:
Culture - CSF (cerebral spinal fluid); Spinal fluid culture

How the test is performed:

The CSF is usually obtained through a lumbar puncture (spinal tap).

The culture is done in the lab. The fluid is placed in special media and allowed to grow. The microbiologist checks it every day. If the cultures grow (a "positive culture"), the microorganisms are identified and antibiotic susceptibility is checked, allowing for the best choice of antibiotics.

How to prepare for the test:

Preparation is the same as for a lumbar puncture. You must sign a consent form. You must be prepared to remain in the hospital for at least the 6 to 8 hours that you must remain lying down.

How the test will feel:

The position may be uncomfortable, but you MUST remain in the curled position to avoid moving the needle and possibly injuring the spinal cord.

The scrub will feel cold and wet. The anesthetic will sting or burn when first injected. There will be a hard pressure sensation when the needle is inserted, and there is usually some brief pain when the needle goes through the meninges (membranes covering the spinal cord). This pain should stop in a few seconds. Overall, discomfort is minimal to moderate.

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